cdna library การใช้

• PVI has been widely used for the display of cDNA libraries.
• Hence, cDNA libraries are a powerful tool in modern research.
• Fast skeletal muscle TnI was first cloned from a skeletal muscle cDNA library.
• Traditional techniques took the whole tissue sample and produced bulk tissue cDNA libraries.
• In 1983, Putney et al . sequenced 178 clones from a rabbit muscle cDNA library
• Seven cDNA libraries are available online.
• In 2000, full length NKX3-1 cDNA was obtained from a human prostate cDNA library.
• PCR can also be used to determine whether a particular DNA fragment is found in a cDNA library.
• The first PHLDB1 cDNA was cloned from a rat pituitary cDNA library and named as LL5 after the clone number.
• Once selected, stocks of the bacteria are created which can later be grown and sequenced to compile the cDNA library.
• A cDNA library is constituted by the cDNA produced through reverse transcription of mRNA collected from specific cells of types of cell.
• The first mRNA encoding the alpha subunit of ENaC was isolated by two independent groups by screening a rat colon cDNA library.
• His lab was the first to use PND patient antisera to screen expression cDNA libraries to identify the genes encoding the PND antigens.
• A mouse embryo cDNA library was screened to identify a RhoA-GTP-binding protein using a yeast two-hybrid system.
• While pVI has been useful for the analysis of cDNA libraries, pIII and pVIII remain the most utilized coat proteins for phage display.
• The nck gene was initially isolated from a human melanoma cDNA library using a monoclonal antibody produced against the human melanoma-associated antigen.
• By screening a cDNA library constructed from human promyelocytic leukemia cells, Parkos et al . isolated a cDNA corresponding to the light chain of cytb.
• The human equivalent was obtained by cDNA library screening using the murine gene as a probe and found to be homologous to the Drosophila awd gene.
• An EST results from one-short sequencing of a cloned cDNA . The cDNAs used for EST generation are typically individual clones from a cDNA library.
• In 1982, the idea of selecting random or semi-random clones from such a cDNA library for sequencing was explored by Greg Sutcliffe and coworkers.